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    The aspartame contained in diet soda stored over time can break down into...poisons.

    "The aspartame contained in diet soda stored over time can break down into
    formaldehyde and DKP, two very dangerous poisons.
    "

    Amazing story. See website here:

    THE EFFECTS OF DIFFERENT STORAGE TEMPERATURES ON THE
    TASTE AND CHEMICAL COMPOSITION OF DIET COKE

    BY JENNIFER COHEN
    -----------------

    Jennifer Cohen is an eleven-year old student in Mrs. Simmons' sixth grade
    Oradell, New Jersey class. The principal of Oradell Public School is
    Scott Ryan. He may be reached at 201 261-1181. Jennifer conducted an
    experiment proving aspartame, the artificial sweetener in diet soda, breaks
    down into two deadly neurotoxins when stored at room temperature and under
    refrigeration.

    -----------------

    ABSTRACT: The level of aspartame in a can of Diet Coke was found to be
    0.06% by a food testing laboratory. The remaining cans from one case of
    Diet coke were stored under three different heat conditions for 10 weeks.
    Seven cans were stored in an incubator (104 degrees Fahrenheit), seven
    cans were stored at room temperature (68-70 degrees Fahrenheit). At the
    end of 70 days samples were tested for levels of aspartame, formaldehyde
    and DKP (diketopiperazine). The refrigerated sample contained 0.058
    percent aspartame, 0.001 percent DKP and 53.5 parts per billion of
    formaldehyde. The room temperature sample contained 0.051 percent
    aspartame, 0.002 percent DKP and 231 parts per billion of formaldehyde.
    The incubator sample contained 0.026 percent aspartame, 0.010 percent DKP
    and 76.2 parts per billion of formaldehyde. In addition 10 human subjects
    tasted each soda sample plus a new can of Diet Coke and rated each sample
    for taste on a 1-4 scale with 1 being the best and 4 being the worst. The
    new can of Diet Coke received an average rating of 2.0. The sample stored
    in the refrigerator received an average rating of 2.6. The sample stored
    at room temperature received an average rating of 2.5. The sample stored
    in the incubator received an average rating of 3.8. The effects of heat
    on Diet coke produced the worst taste and the highest amount of loss of
    aspartame as well as the greatest increase in levels of DKP. The most
    pleasing taste was for the new can of Diet Coke. The room temperature
    sample and the refrigerated sample scored almost the same in the taste
    test. All samples revealed a presence of formaldehyde. However, the
    highest level of formaldehyde occurred in the room temperature can.
    There was also formaldehyde present in the refrigerated sample.

    BACKGROUND: Aspartame was discovered in l965 by Searle chemist, Jim
    Schlatter. He was developing this drug for another use and after
    accidentally licking his finger found that aspartame was sweet. Today
    aspartame is consumed by more than 100 million people in the United
    States. This chemical (aspartame) has been approved by the Food and Drug
    Administration (FDA) who said that an individual can safely consume 97
    packets of aspartame every day. Aspartame is in many products including
    some that children use such as diet soda, light yogurt, Flintstone
    Vitamins, baked goods, puddings, and Winterfresh gum. It has been known
    to cause headaches, nausea, vision problems, seizures and cancer in its
    users.

    The ingredients in aspartame are aspartic acid, phenylalanine, and methyl
    alcohol. Methyl alcohol is a chemical that breaks down in high
    temperatures and turns into formaldehyde and DKP (diketopiperazine), two
    chemicals known to cause problems in the nervous system. Aspartame's life
    is 262 days at 77 degrees Fahrenheit, or 25 degrees Celsius. The FDA gets
    more complaints about aspartame than any other food or drink. The
    symptoms of aspartame are a lot like the symptoms of multiple sclerosis
    and Alzheimer's disease. Ever since aspartame was approved in l985, there
    has been an increase in brain tumors. There is no direct proof that
    aspartame caused the brain tumors, but there is enough reason to suspect
    that, and the television show, "60 Minutes" recently did a report linking
    the increase in brain cancer to aspartame use.

    The FDA reviewed Searle's studies of this artificial sweetener in which
    rats were fed aspartame daily with their meals for one year. There were
    12 brain tumors in the 320 rats that were fed aspartame and no brain
    tumors in the 120 rats that were not fed aspartame.

    There was also a study done at the University of Wisconsin on rhesus
    monkeys. they were fed aspartame daily. After day 200 of a one year
    study the monkeys developed epileptic seizures. After the study ended,
    the aspartame was discontinued and the monkeys were fully watched for
    60 days. The monkeys had no more seizures.

    METHOD: I did my own experiment on aspartame. On January 21, l997, I
    bought a new case of Diet Coke from the supermarket. I put 7 cans in the
    refrigerator, 7 cans in my room at room temperature (about 69 degrees) and
    I put 7 cans in a BOEKEL incubator (80 Watts, 120 AC volts, 0.75 Amps,
    catalog # 131500) and set the temperature at 40 degrees Celsius which is
    104 degrees Fahrenheit. I left them in there for 10 weeks (70 days). I
    had a thermometer next to each group of cans and I checked the
    temperatures daily. I took the remaining three cans and brought them to
    Winston Laboratories in Ridgefield, New jersey to test for a beginning
    level of aspartame. When I got the test results back, they revealed that
    there was normally 0.06 per cent of aspartame in the can of diet soda.

    I chose that temperature because in l985 the National Soft Drink
    Association reported a similar experiment in which diet soda stored at
    that temperature turned into formaldehyde. In that experiment they
    explained that 104 degrees Fahrenheit was equal to a daytime temperature
    in Phoenix, Arizona over the summer. The National Soft Drink Association
    recommended that aspartame not be approved for use by people in soda.
    They published this experiment and their recommendation in the
    Congressional Record.

    On April 1, I took the cans of aspartame out of the refrigerator, out of
    my room and out of the incubator. That day I brought the samples to
    Winston Laboratory for analysis.

    I was going to do a taste in my sister's fourth grade class, but the
    school nurse said that I couldn't because of all the bad things people say
    about aspartame, so instead I tested the samples on a group of adults.

    I performed a double blind experiment. My mother helped to label each
    sample with a number. I conducted the experiment but I did not know which
    sample each person was drinking. I put all of the cans in a cooler and
    covered them with ice sos that they would be served at the same
    temperature. I gave each person a small cup of the soda from the
    refrigerator, from the incubator, from my room, and from a new can of soda
    fresh from the supermarket. I asked them to rate the taste on a scale of
    one to four, four being the worst and one being the best. The actual
    results are to be found in table#1.

    Table 1

    ROOM COLD WARM NEW
    TEMP. STORAGE STORAGE CAN
    SAMPLE SAMPLE SAMPLE SAMPLE
    #517 #502 #540 #563

    SUBJECT #1 4 4 4 3
    SUBJECT #2 3 2 4 1
    SUBJECT #3 2 3 3 2
    SUBJECT #4 1 2 4 2
    SUBJECT #5 2 2 4 1
    SUBJECT #6 2 3 4 1
    SUBJECT #7 2 3 3 4
    SUBJECT #8 3 2 4 3
    SUBJECT #9 3 2 4 1
    SUBJECT #10 3 3 4 2

    AVERAGE 2.5 2.6 3.8 2.0


    Table #2 contains the results of the testing conducted on the samples
    analyzed by Winston Laboratories. In the sample that was in the
    refrigerator all that was left of the 0.06 per cent of the aspartame was
    0.058 percent. That extra aspartame had turned into 0.001 percent DKP and
    53.5 parts per billion of formaldehyde In the sample from my room, all
    that was left of the 0.06 percent aspartame was 0.051 per cent. The extra
    aspartame had turned into 0.002 percent DKP and 231 parts per billion of
    formaldehyde. In the sample that was in the incubator all that was left
    of the 0.06 percent aspartame was 0.026 percent. The extra aspartame had
    turned into 0.010 percent DkP and 76.2 parts per billion in the
    formaldehyde.

    Table 2

    ASPARTAME DKP FORMALDEHYDE
    % % Parts per billion

    BASELINE CAN 0.060% * *


    SAMPLE # 502 0.058% 0.001% 53.5
    (refrigerated)


    SAMPLE # 517 0.051% 0.002% 231.0
    (room temperature)


    SAMPLE #540 0.026% 0.010% 76.2
    (incubator)


    SAMPLE # 563 * * *
    (new can)

    * Sample #563 (new can of Diet Coke was not tested by the lab. It was
    used for the taste test only. The baseline can was not tested for
    formaldehyde or DKP because it was assumed that FDA would ban any new
    product containing poison. The total cost of testing was $1250. This
    may not be a lot of money to a drug company but it is to me. As it is,
    I will be baby-sitting for the summer of 1997 to pay for this study. -JC


    DISCUSSION: There was an obvious preference for the newly purchased
    sample of Diet Coke. The scores for the sample in the refrigerator and
    room temperature were similar but were not as high as the new soda. The
    score for the incubator sample were very low. Nearly everybody hated the
    taste.

    There are taste differences. Ten people preferred the new soda to the
    other three samples. With 1 being the best and 4 being the worst, their
    average score for the new soda was 2.0.

    The aspartame contained in diet soda stored over time can break down into
    formaldehyde and DKP, two very dangerous poisons. Taste tests revealed a
    noticeable difference among tasters. The higher the heat of storage, the
    worse the taste.

    Diet soda stored for ten weeks loses flavor. Aspartame in that soda
    breaks down into two products, formaldehyde and DKP. The warmer the
    temperature, the greater the loss of aspartame and the greater the
    increase in DKP.

    Temperature creates two effects. First, the higher the temperature of
    storage, the higher the level of DKP in the soda. Second, room
    temperature seems to create the highest levels of formaldehyde in soda.
    At very high temperatures, the formaldehyde breaks down. However, even
    stored in a refrigerator at cold temperature, the aspartame breaks down
    into formaldehyde.

    After diet soda containing aspartame is purchased it should not be stored
    in the heat or under any condition for a long period of time. Further
    research should be performed with more samples at different temperatures
    for different time periods so that safety levels can be determined.

    Concerning aspartame, the FDA says, "we believe that based on all the
    information that we received to date that this is a safe product." I
    say, "Decide for yourself." Jennifer Cohen


    Bibliography

    1) Artificial Sweeteners. "The World Book Encyclopedia" 1997
    2) Azarnoff, Daniel. "Aspartame" McGraw-Hill.
    Encyclopedia of Science and Technology 1987
    3) Constantine, Alex. "The NutraPoison"
    http://www.dorway.com/possible.html (items 26 and 27) March 13, 1997
    4) Jibrin, Janis. "How Sweet It Is."
    American Health December 1995: 84-85
    5) Jibrin, Janis. "Is Sugar Poison?"
    Mademoiselle December 1995: 84-85
    6) Lepke, Janet. "A Dieter's Guide To Sugar Substitutes."
    Consumer's Digest May/June 1995: 71-74
    7) Rao, K.S., McConnell, R.G., Waisman, H.A. SC-18862
    52 Week Oral Toxicity Study In The Infant Monkey
    8) Roberts, H.J. Aspartame (Nutra Sweet) Is It Safe?
    Philadelphia: The Charles Press, 1990]
    9) Roberts, H.J. Sweetener Dearest. West Palm Beach:
    The Sunshine Sentinel Press, 1992